Irf3 phosphorylation antibody
WebDec 15, 2014 · To directly observe the distribution of HSPD1 and activated IRF3, an antibody specific to phosphorylated IRF3 (phospho S386) was used. Not surprisingly, overexpression of MAVS for 16 h resulted in phosphorylation of IRF3. Furthermore, almost all of the activated IRF3 co-localized with HSPD1 at that time point . WebNational Center for Biotechnology Information
Irf3 phosphorylation antibody
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WebApr 14, 2003 · Viral infection results in the phosphorylation of IRF3, leading to its dimerization and interaction with the coactivators CREB-binding protein (CBP) and p300 … WebAll mutants lacked phosphorylation of the STING-S365 residue, IRF3, and STAT1 upon stimulation, indicating defects in IRF3 activation and type I IFN signaling (Fig. 1A and SI Appendix, Fig. S1A). Consistently, all mutants were deficient in IRF3 nuclear translocation ( Fig. 1 B and SI Appendix , Fig. S1 B ) and did not produce IFNβ after DMXAA ...
WebMar 6, 2012 · Aliquots of the cell lysates were analyzed by Western blotting with an antibody against IRF3 after native PAGE (top). Data are from a single experiment representative of … WebPhospho-IRF3 (Ser396) Antibody (MA5-14947) in WB Frontiers in microbiology 2024 - FIGURE 2 Effects of Set-Met on the expression of MAVS protein and the phosphorylation of IRF-3 induced by PDCoV. LLC-PK cells were incubated with PDCoV for 1 h, and 2, 4, 8, and 16 muM of Se-Met was added.
WebAnti-IRF3 antibody [IRF35I218] (ab50772) Datasheet Reviews ( 3) Q&A (4) References (9) Key features and details Mouse monoclonal [IRF35I218] to IRF3 Suitable for: ICC/IF, WB, Flow Cyt Reacts with: Human Isotype: IgG1 You may also be interested in Primary Alexa Fluor® 488 Anti-PSMA antibody [GCP05] (ab187570) WebCustom Monoclonal Antibodies; CellPower™ Assay Cell Line Development; DIAGNOSTICS Hot! COVID-19. cPass™ Technology . Rapid Tests. ... Tyrosine phosphorylation of the E3 ubiquitin ligase TRIM21 positively regulates interaction with IRF3 and hence TRIM21 activity. PLoS One.. 2012-03; 7(3): ...
WebAll mutants lacked phosphorylation of the STING-S365 residue, IRF3, and STAT1 upon stimulation, indicating defects in IRF3 activation and type I IFN signaling (Fig. 1A and SI …
WebOct 22, 2024 · Phosphorylation occurs when IRF3 is recruited to adaptor proteins via a positively charged surface within the regulatory domain of IRF3. This positively charged surface also plays a crucial role in forming the active homodimer by interacting with the phosphorylated sites stabilizing the homodimer. flex seal instructions drying times etcWebMar 5, 2024 · Rnf39 deficiency and knockdown both enhanced RNA virus–induced IRF3 phosphorylation (Fig. 2, A to C, and fig. S3D). Furthermore, ... The cell lysates were immunoprecipitated with Flag antibody and then immunoblotted with the indicated antibodies. The specific bands were marked by asterisk. All experiments were repeated at … flexseal internal push fit couplingWeb), the data reported here suggest that additional signals stimulate phosphorylation of IRF3 and IRF7, essential events in induction of the protective IFN response. This evidence is … chelsea taped lounge setWebMar 21, 2024 · IRF3 (Interferon Regulatory Factor 3) is a Protein Coding gene. Diseases associated with IRF3 include Encephalopathy, Acute, Infection-Induced 7 and Encephalopathy, Acute, Infection-Induced 10 . Among its related pathways are Cytosolic sensors of pathogen-associated DNA and MyD88 dependent cascade initiated on … chelsea tattooWebApr 11, 2024 · Since EhaF-mediated inhibition of TLR4-TRIF-IFN pathway occurs at the level of IRF3 phosphorylation ... Cells were lysed after 1.5 h and IRF3 was immunoprecipitated with anti-IRF3 antibody (1:100 ... chelsea tattoo ideasWebMar 4, 2024 · IRF3 is a transcriptional factor that mediates expression of a large array of genes after viral infection. We hypothesized that DUBs might regulate the activity or stability of IRF3 and thereby facilitate or inhibit cellular antiviral responses. flex seal is it any goodWebIRF3 and IRF7 Phosphorylation in Virus-infected Cells Does Not Require Double-stranded RNA-dependent Protein Kinase R or IκB Kinase but Is Blocked by Vaccinia Virus E3L Protein* - Journal of Biological Chemistry MECHANISMS OF SIGNAL TRANSDUCTION Volume 276, ISSUE 12, P8951-8957, March 2001 chelsea tattoo shop